A Novel Vector System for Rapid Development of Knock-in Cell Lines With CRISPR

A Novel Vector System for Rapid Development of Knock-in Cell Lines With CRISPR

04/28/2020, 11:30 AM - 12:15 PM

Contract/Chemspec USA Stage, Booth 1058, Exhibit Hall
Language:
English
While CRISPR/Cas9 greatly facilitates gene editing, its use for tagging endogenous proteins via reporter gene knock-in remains constrained because the process is tedious and time-consuming. Here we describe a homologous recombination system (FAST-HDR) that, in combination with CRISPR/Cas9, makes this process far more efficient. We developed advanced cell lines with as many three fluorescently tagged endogenous proteins, permitting longitudinal high-content phenotype- or target-based drug screening and obviating the need for fixation, staining, and immunofluorescence. Thus, the FAST-HDR system can be used for developing enhanced cell-based models for drug discovery and other biologic research.

Contributors

  • Oscar Perez-Leal, MD

    Speaker

    Assistant Professor

    Temple University School of Pharmacy

    Dr. Perez is an Assistant Professor in the Pharmaceutical Sciences Department of the School of Pharmacy at Temple University. Dr. Perez's expertise...

Type of Session

  1. Type of Session
    Session

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